National Institute of Plant Genome Research
Digital India   Azadi Ka Amrit Mahotsav     
 
    Dr. Sabhyata Bhatia, FNA, FNASc
    Chair Scientist
    Tel: 91-11-26741612,14,17 Ext. - 159, 232
    Direct - 26735159/26735232/ 9899101387
    E-mail: sabhyatabhatia@nipgr.ac.in, sabhyatab@yahoo.com

 Honours

Fellow, Indian National Academy of Sciences (INSA), India (2024).

Fellow,The National Academy of Sciences (NASI), India (2014).

 Research Area

Generating genomic resources for gene discovery and crop improvement

 Research Interests

  1. Utilizing integrated genomic approaches to identify and characterize novel loci/genes governing seed yield and nutritional traits in legumes (Chickpea, Lentils, Cowpea).
  2. Delineating molecular mechanisms regulating seed storage compound accumulation in chickpea and lentil.
  3. Generating genomic resources in chickpea, lentil, cowpea and black gram.
  4. Dissecting salinity stress tolerance in lentil.
  5. Dissecting heat stress tolerance in lentil.
  6. Exploring the mechanisms underlying chickpea nodulation.

 Research Highlights

The goal has been to analyse plant genomes to facilitate breeders with new tools for improving plant production and nutrition. Several innovative concepts, methods, and analyses were tailored to the experimental objectives and implemented (several for the first time in the country). Pioneering work has been done in generating high throughput genomic resources (SSRs, SNPs, ESTs, linkage maps, QTLs, whole genome and transcriptome sequences) in legumes such as chickpea, lentil, and minor pulses that were used to study genome structure and evolution, molecular diversity, variety identification, high-resolution genome mapping, gene mapping, and trait association analysis. Significant contributions include the cracking of the whole genome sequence of Chickpea, Black gram and Cowpea.
Using integrated genomic techniques such as whole genome resequencing-based QTL-Seq and Genome Wide Association mapping study (GWAS), novel QTLs and markers tightly related with seed protein content in chickpea have been identified for application in marker assisted breeding. Similarly, integrated transcriptomics and genotyping-by-sequencing (GBS) were used to identify QTLs and candidate genes responsible for seed size, weight, and Fe content in chickpea and lentil, as well as rust resistance in lentils. In minor pulses, high quality chromosome level genome assemblies have been generated for Vigna mungo (black gram) and V. unguiculata (cow pea) alongwith tissue-specific deep transcriptomes for creating the 'gene expression atlas'.

Work is also focussed on dissecting chickpea's two hallmark traits: (i) seed traits for increased yield and nutrition and (ii) nodulation traits for increased nitrogen fixation. Reverse genetics techniques were used to comprehend the complex gene-regulatory networks that control seed storage protein (SSP) synthesis in chickpea wherein the novel role of Ca-miR164e- CaNAC100 module as well as that of CaREN1 and its interacting protein partners in regulating seed storage protein synthesis in chickpea was established. Furthermore, with the aim of enhancing the nitrogen fixation efficiency by increasing the nodule numbers, several genes and miRNAs have been characterized to get insights into the regulation of early nodulation events in chickpea.
Currently the group envisages comprehending the complex genetic and molecular basis of key nutritional traits in the important legumes i.e.lentil and chickpea. Utilizing the expertise of genomics and plant transformation, it is proposed to identify and functionally validate through transgenesis and gene editing techniques, the novel genes/loci associated with seed protein content, carotenoid, seed iron and zinc content as well as traits pertaining to yield, heat and salinity stress in order to address the global demand of nutrient enriched crop varieties.

 Insilico Resources Developed

  1. Whole genome sequencing and assembly of chickpea and black gram
  2. Deep transcriptome of developing seeds of chickpea
  3. Deep transcriptome of developing nodules of chickpea
  4. Deep transcriptome atlas of lentil
  5. Transcriptome of chickpea under iron starvation
  6. Transcriptome of lentil under iron stress
  7. miRNAs from chickpea nodules7. miRNAs from chickpea nodules
  8. miRNAs under iron stress in lentil
 Group Members:
 Ongoing projects
 Recent Publications
Chakraborty A., Singh B., Pandey V., Parida SK., Bhatia S (2024). MicroRNA164e suppresses NAC100 transcription factor mediated synthesis of seed storage proteins in chickpea. New Phytologist doi:10.1111/nph.19770
Singh, B., Singh, S., Mahato, A.K., Dikshit, H.K., Tripathi, K. and Bhatia, S., (2023) Delineation of novel genomic loci and putative candidate genes associated with seed iron and zinc content in lentil (Lens culinaris Medik.). Plant Science, 335, p.111787.
Junaid A, Singh B and Bhatia S. (2023) Evolutionary insights into 3D genome organization and epigenetic landscape of Vigna mungo. Life Science Alliance Vol7, no.1 e202302074 (DOI:10.26508/lsa.202302074)
Chakraborty A, Junaid A, Parida SK, Bhatia S (2023) Integrated genomic approaches delineate a novel role of ROP1 ENHANCER1 in controlling seed protein content of chickpea. J Exp Bot 74 (3): 817-834 (doi.org/10.1093/jxb/erac452)
Singh, G., Ambreen, H., Jain, P., Chakraborty, A., Singh, B., Manivannan, A., Bhatia, S. (2023) Comparative transcriptomic and metabolite profiling reveals genotype-specific responses to Fe starvation in chickpea. Physiologia Plantarum, 175( 2), e13897. https://doi.org/10.1111/ppl.13897
Padhy A.K, Kaur P, Singh B, Kaur R, Bhatia S, Shamshad M, Sharma H, Kaur S, Srivastava P & Sharma A (2023) In silico characterization of Thinopyrum elongatum-derived PsyE1 gene and validation in 7D/7E bread wheat introgression lines open avenues for carotenoid biofortification in wheat. CEREAL RESEARCH COMMUNICATIONS 51, 75-85. https://doi.org/10.1007/s42976-022-00279-w
Tiwari M, Yadav M, Singh B, Pandey V, Nawaz K, and Bhatia S (2021) Evolutionary and functional analysis of Two-Component System in chickpea reveals CaRR13, a TypeB RR, as positive regulator of symbiosis. Plant Biotechnol. J., https://doi.org/10.1111/pbi.13649
Tiwari M, Pandey V, Singh B, Yadav M, Bhatia S* (2021). Evolutionary and expression dynamics of LRR-RLKs and functional establishment of KLAVIER homolog in shoot mediated regulation of AON in chickpea symbiosis. Genomics: https://doi.org/10.1016/j.ygeno.2021.11.022
Tiwari M, Singh B, Yadav M, Pandey V, Bhatia S (2021) High throughput identification of miRNAs reveal novel interacting targets regulating chickpea-rhizobia symbiosis. Environmental and Experimental Botany 186 (104469)
Pradhan S, Verma S, Chakraborty A, Bhatia S (2021) Identification and molecular characterization of miRNAs and their target genes associated with seed development through small RNA sequencing in chickpea. Funct Integr Genomics: 21(2):283-298. doi: 10.1007/s10142-021-00777-w. Epub 2021 Feb 25. PMID: 33630193
Tiwari M, Pandey V, Singh B, Bhatia S (2020) Dynamics of miRNA mediated regulation of legume symbiosis. Plant Cell Environ. https://doi.org/10.1111/pce.13983
Gaur R, Verma S, Pradhan S, Ambreen H, Bhatia S (2020) A high density SNP-based linkage map using Genotyping-by-Sequencing and its utilization for improved genome assembly of chickpea (Cicer arietinum L.). Funct Integr Genomics DOI 10.1007/s10142-020-00751-y
Tiwari M, Bhatia S. (2019) Expression profiling of miRNAs indicates crosstalk between phytohormonal response and rhizobial infection in chickpea. J. Plant Biochem. Biotechnol. https://doi.org/10.1007/s13562-019-00545-9
Verma S, Bhatia S (2019) A comprehensive analysis of the B3 superfamily identifies tissue-specific and stress-responsive genes in chickpea (Cicer arietinum L.) 3 Biotech (2019) 9: 346. https://doi.org/10.1007/s13205-019-1875-5
Verma S & Bhatia S (2018) Analysis of genes encoding seed storage proteins (SSPs) in chickpea (Cicer arietinum L.) reveals co-expressing transcription factors and a seed-specific promoter. Functional & Integrative Genomics: doi.org/10.1007/s10142-018-0650-8 (Accepted)(IF: 3.9)
Berger A, Brouquisse R, Pathak PK, Hichri I, Singh I, Bhatia S, Boscari A, Igamberdiev AU, Gupta KJ (2018) Pathways of nitric oxide metabolism and operation of phytoglobins in legume nodules: Missing links and future directions. Plant Cell Environ.; 1-12. https://doi.org/10.1111/ pce.13151 (IF: 6.173)
Pradhan S, Kant C, Verma S, Bhatia S (2017) Genome-wide analysis of the CCCH zinc finger family identifies tissue specific and stress responsive candidates in chickpea (Cicer arietinum L.). PLoS ONE 12(7): e0180469. https://doi.org/10.1371/journal.pone.0180469  (IF: 3.0)
Kumar S and Bhatia S (2016) A polymorphic (GA/CT)n- SSR influences promoter activity of Tryptophan decarboxylase gene in Catharanthus roseus L. Don. Scientific Reports 6: 33280.
Kant C, Pradhan S and Bhatia S (2016) Dissecting the root nodule transcriptome of chickpea (Cicer arietinum L.). PLoS One doi: 10.1371/journal.pone.0157908 (IF: 3.0)
Garg R, Shankar R, Thakkar B, Kudapa H, Krishnamurthy L, Mantri N, Varshney R, Bhatia S & Jain M (2016) Transcriptome analyses reveal genotype- and developmental stage-specific molecular responses to drought and salinity stresses in chickpea.  Scientific Reports | 6:19228 | DOI: 10.1038/srep19228 (IF: 5.2)
Verma S, Gupta S, Bandhiwal N, Kumar T, Bharadwaj C & Bhatia S (2015) High-density linkage map construction and mapping of seed trait QTLs in chickpea (Cicer arietinum L.) using Genotyping-by-Sequencing (GBS). Scientific Reports 5, 17512; doi: 10.1038/srep17512. (IF: 5.2)
Gupta S, Kumar T, Verma S, Bharadwaj C & Bhatia S. (2015) Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height. Mol Biol Rep, 1-10, doi: 10.1007/s11033-015-3925-3. (IF: 1.68)
Verma P, Goyal R, Chahota RK, Sharma TR, Abdin MZ & Bhatia S (2015) Construction of a genetic linkage map and identification of QTLs for seed weight and seed size traits in lentil (Lens culinaris Medik.) PlosOne, DOI:10.1371/journal.pone.0139666 October 5, 2015 (IF: 3.0)
Gaur R, Jeena G, Shah N, Gupta S, Pradhan S, Tyagi AK, Jain M, Chattopadhyay D & Bhatia S (2015): High density linkage mapping of genomic and transcriptomic SNPs for synteny analysis and anchoring the genome sequence of chickpea. Scientific Reports 5, 13387; doi: 10.1038/srep 13387. (IF : 5.2 )
Parween S, Nawaz K, Roy R, Pole AK, Suresh BV, Misra G, Jain M, Yadav G, Parida SK, Tyagi AK, Bhatia S & Chattopadhyay D (2015) An advanced draft genome assembly of a desi type chickpea (Cicer arietinum L.). Scientific Reports 5, 12806; doi: 10.1038/srep12806. (IF : 5.2)
Kumar S, Bhatia S (2015) Isolation of Catharanthus roseus (L.) G. Don nuclei and measurement of rate of Tryptophan decarboxylase gene transcription using nuclear run-on transcription assay. PlosOne, DOI:10.1371/journal.pone.0127892 May 29, 2015. (IF : 3.0)
Kumar T, Bharadwaj C, Alam A, Singh P, Singhal T, Chauhan SK, Bhatia S, Sarker A (2015) Development and characterization of Recombinant Inbred Lines for seed related traits in chickpea. IJTA, 33(2): 639 – 642. (NAAS rating 3.03)
Khajuria YP, Saxena , M.S., Gaur, R., Chattopadhyay, D., Jain, M., Parida, S.K., Bhatia, S. (2015) Development and integration of genome-wide polymorphic microsatellite markers onto a reference linkage map for constructing a high-density genetic map of chickpea. PlosOne, DOI: 10.1371/journal.pone.0125583. (IF : 3.6)
Gupta S, Garg V, Bhatia S (2015): A new set of ESTs from chickpea (Cicer arietinum L.) embryo reveals two novel F-box genes, CarF-box_PP2 and CarF-box_LysM, with potential roles in seed development. Plos One (DOI: 10.1371/journal.pone.0121100) (IF : 3.0)
Gupta S, Garg V, Kant C, Bhatia S (2015): Genome-wide survey and expression analysis of F-box genes in chickpea. BMC Genomics (DOI: 10.1186/s12864-015-1293-y). (IF: 3.8)
Verma P, Chandra A, Roy AK, Malaviya D R, Kaushal P, Pandey D, Bhatia S (2015): Development, characterization and cross-species transferability of genomic SSR markers in berseem (Trifolium alexandrinum L.), an important multi-cut annual forage legume. Molecular Breeding (DOI 10.1007/s11032-015-0223-7). (IF: 2.1)
Pradhan S, Bandhiwal N, Shah N, Kant C, Gaur R, Bhatia S (2014): Global transcriptome analysis of developing chickpea (Cicer arietinum L.) seeds. Frontiers in Plant Science Vol 5, Article 698 (doi: 10.3389/fpls.2014.00698) . (IF: 4.59)
Misra G, Priya P, Bandhiwal N, Bareja N, Jain M, Bhatia S, Chattopadhyay D, Tyagi AK, Yadav G (2014): The Chickpea Genomic Web Resource: Visualization and Analysis of the Desi-type Cicer arietinum Nuclear Genome for Comparative Exploration of Legumes. BMC Plant Biology (DOI: 10.1186/s12870-014-0315-2). (IF: 3.6)
Verma P, Sharma TR, Srivastava PS, Abdin MZ, Bhatia S (2014) Exploring genetic variability within lentil (Lens culinaris Medik.) and across related legumes using a newly developed set of microsatellite markers. Mol Biol Rep. 41(9):5607-25 (DOI 10.1007/s11033-014-3431-z). (IF: 1.69)
Shrivastava D, Verma P, Bhatia S (2014) Expanding the repertoire of microsatellite markers for polymorphism studies in Indian accessions of mung bean (Vigna radiata L. Wilczek). Mol Biol Rep. 41(9):5669-80   (DOI 10.1007/s11033-014-3436-7). (IF: 1.69)
Kumar S, Shah N, Garg V, Bhatia S (2014) Large scale in-silico identification and characterization of simple sequence repeats (SSRs) from de novo assembled transcriptome of Catharanthus roseus (L.) G. Don. Plant Cell Reports 33:905–918 (DOI 10.1007/s00299-014-1569-8). (IF: 3.08)
Gaur R, Bhatia S, Gupta M (2014) Generation of expressed sequence tags under cadmium stress for gene discovery and development of molecular markers in chickpea. Protoplasma 251: 955-72.  (DOI: 10.1007/s00709-013-0609-5). (IF: 2.34)
Varshney RK, Mir RR, Bhatia S, Thudi M, Hu Y, Azam S, Zhang Y, Jaganathan D, You FM, Gao J, Riera-Lizarazu O, Luo M-C (2014) Integrated physical, genetic and genome map of chickpea (Cicer arietinum L.) Funct Integr Genomics 14(1):59-73 (DOI 10.1007/s10142-014-0363-6). (IF: 2.26)
Verma P, Shah N and Bhatia S (2013) Development of an expressed gene catalogue and molecular markers from the de novo assembly of short sequence reads of the lentil (Lens culinaris Medik.) transcriptome. Plant Biotech. J. 11: 894-905  (doi: 10.1111/pbi.12082). (IF: 6.09)
Jain M, Misra G, Patel RK, Priya P, Jhanwar S, Khan AW, Shah N, Singh VK, Garg R, Jeena G, Yadav M, Chandra Kant, Sharma P, Yadav G, Bhatia S, Tyagi AK, Chattopadhyay D (2013) A draft genome sequence of the pulse crop chickpea (Cicer arietinum L.). Plant J. 74: 715-729 (DOI: 10.1111/tpj.12173). (IF: 6.28)
Gaur R, Azam S, Jeena G, Khan AW, Choudhary S, Jain M, Yadav G, Tyagi AK, Chattopadhyay D, Bhatia S (2012) High-throughput SNP discovery and genotyping for constructing a saturated linkage map of chickpea (Cicer arietinum L.). DNA Research, 19:357–373 (doi: 10.1093/dnares/dss018). (IF: 5.47)
Choudhary S, Gaur R., Gupta S, Bhatia S (2012) EST-derived genic molecular markers: development and utilization for generating an advanced transcript map of chickpea. Theor. Appl. Genet. 124: 1449-1462 (DOI 10.1007/s00122-012-1800-3). (IF: 3.9)
Gupta M, Verma B, Kumar N, Chahota RK , Rathour R, Sharma SK , Bhatia S,  Sharma TR (2012)  Construction of intersubspecific molecular genetic map of lentil based on ISSR, RAPD and SSR markers. Jour. Genet. 12, 91(3):279-87. (IF: 1.1)
Shokeen B, Choudhary S, Sethy NK, Bhatia S (2011) Development of SSR and gene-targeted markers for construction of a framework linkage map of Catharanthus roseus. Ann. Bot., London, 108, 321–336. (IF: 4.0)
Garg R, Patel RK, Jhanwar S, Priya P, Bhattacharjee A, Yadav G, Bhatia S, Chattopadhyay D, Tyagi AK, Jain M (2011) Gene discovery and tissue-specific transcriptome analysis in chickpea with massively parallel pyrosequencing and web resource development, Plant Physiol., 156: 1661–1678. (IF: 8.03)
Chaudhary S, Sharma V, Prasad Manoj, Bhatia S, Tripathi BN, Yadav Gitanjali, Kumar S (2011). Characterization and genetic linkage mapping of the horticulturally important mutation leafless inflorescence (lli) in periwinkle Catharanthus roseus. Scientia Horticulturae 129: 142-153, DOI : 10.1016/j.scientia.2011.02.025. (IF: 1.53)
Gaur R, Sethy NK, Choudhary S, Gupta V, Shokeen B, Bhatia S (2011) Advancing the STMS genomic resources and defining their locations on the intra-specific genetic linkage map of chickpea (Cicer arietinum L.) BMC Genomics 12: 117. (IF: 4.4)
Gujaria N, Kumar A, Dauthal P, Dubey A, Hiremath P, Bhanu Prakash A, Farmer A, Bhide M, Shah T, Gaur P, Upadhyaya H D, Bhatia S, Cook D R, May G D, Varshney R K (2011) Development and use of genic molecular markers (GMMs) for constructing a transcript map of chickpea (Cicer arietinum L.) Theor. Appl. Genet. 122: 1577-1589 (DOI 10.1007/s00122-011-1556-1). (IF: 3.86)
Gupta V, Bhatia S, Mohanty NA, Sethy N, Tripathy BC (2010) Comparative analysis of photosynthetic and biochemical characteristics of Desi and Kabuli genepools of chickpea (Cicer arietinum L.) Int. Jour of Genetic Engineering and Biotechnology. Vol 1, Num 1, 65 – 76.
Choudhary S, Sethy NK, Shokeen B, Bhatia S (2009) Development of chickpea EST-SSR markers and analysis of allelic variation across related species. Theor Appl Genet 118:591–608 (DOI10.1007/s00122-008-0923-z).   (IF: 3.9)
Shokeen B, Sethy NK, Kumar S, Bhatia S (2007) Isolation and characterization of microsatellite markers for analysis of molecular variation in the medicinal plant Madagascar periwinkle (Catharanthus roseus (L.) G. Don.). Plant Science 172: 441 – 451. (IF: 3.36)
Sethy NK, ShokeenB, Edwards KJ, Bhatia S (2006) Development of microsatellite markers and analysis of intraspecific genetic variability in chickpea (Cicer arietinum L.). Theor. and Appl. Genet. 112:1426 -1428. (IF: 3.9)
Sethy NK, Choudhary S, Shokeen B, Bhatia S (2006): Identification of microsatellite markers from Cicer reticulatum: molecular variation and phylogenetic analysis. Theor. Appl. Genet. 112: 347-357. (IF: 3.9)
Choudhary S, Sethy NK, Shokeen B, Bhatia S (2006): Development of sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.). Mol Ecol. Notes 6(1): 93-95. (IF: Currently Mol. Ecol.Res. : 5.3)
Shokeen B, Sethy NK, Choudhary S, Bhatia S (2005): Development of STMS markers from the medicinal plant Madagascar periwinkle (Catharanthus roseus (L.) G. Don.) Mol Ecol. Notes 5, 818-820 (IF: Currently Mol. Ecol.Res. : 5.3)
Sethy NK, Shokeen B, Bhatia S (2003): Isolation and characterization of sequence-tagged microsatellite sites (STMS) markers in chickpea (Cicer arietinum L.). Mol Ecol. Notes, 3, 428-430. (IF: Currently Mol. Ecol.Res. : 5.3)
Rajagopal J, Bashyam L, Bhatia S, Khurana DK, Srivastava PS, Lakshmikumaran M (2000): Evaluation of genetic diversity in the Himalayan Poplar using RAPD markers. Silvae Genetica 49(2): 60-66
Singh A, Negi MS, Rajagopal J, Bhatia S, Tomar UK, Srivastava PS, Lakshmikumaran M (1999). Assessment of genetic diversity in Azadirachta indica using AFLP markers. Theor Appl Genet. 99(1/2): 272-279
Das S, Rajagopal J, Bhatia S, Srivastava PS, Lakshmikumaran M (1999). Assessment of genetic variation within Brassica campestris cultivars using AFLP and RAPD markers. J. of Biosciences 24(4), 433-440.
Bhatia S, Negi MS, Lakshmikumaran M (1996) Structural Analysis of the rDNA Intergenic Spacer of Brassica nigra: Evolutionary Divergence of the Spacers of the Three Diploid Brassica Species. Journal of Molecular Evolution 43(5): 460-468.
Bhatia S, Das S, Jain A, Lakshmikumaran M (1995) DNA fingerprinting of Brassica juncea cultivars using microsatellite probes. Electrophoresis 16: 1750-1754
Jain A, Bhatia S, Banga SS, Prakash S, Lakshmikumaran M (1994) Potential use of the random amplified polymorphic DNA (RAPD) to study the genetic diversity in Indian mustard (Brassica juncea) and its relationship to heterosis. Theor Appl Genet 88: 116-122.
Singh K, Bhatia S, Lakshmikumaran M (1994) Novel variants of the 5S rRNA genes in Eruca sativa. Genome 37: 121-128
Bhatia S, Singh K, Jagannathan V, Lakshmikumaran M (1993): Organization and analysis of the 5S rRNA genes in B. campestris. Plant Science 92: 47-55
  
  For more publication click here
https://scholar.google.com/citations?user=8yGjudwAAAAJ&hl=en&oi=ao
 Book Chapters:
Padhy, A. K., Singh, B., and Bhatia, S. (2023). Understanding Fungal Diseases and Their Mitigation in Lentils. In Diseases in Legume Crops: Next Generation Breeding Approaches for Resistant Legume Crops (pp. 257-281). Singapore: Springer Nature Singapore.
Singh, B., Padhy, A. K., Ambreen, H., Yadav, M., Bhardwaj, S., Singh, G., Pandey, V., Chakraborty, A. and Bhatia S. (2022). Understanding Abiotic Stress Responses in Lentil Under Changing Climate Regimes. In: Jha, U.C., Nayyar, H., Agrawal, S.K., Siddique, K.H.M. (eds) Developing Climate Resilient Grain and Forage Legumes. Springer, Singapore. Pp 179-204. https://doi.org/10.1007/978-981-16-9848-4_9
Dhakate P., Ambreen H., Chakraborty A., Singh B., Yadav M., Tiwari M., Singh G., Pandey V. and Bhatia S. (2020) Comprehending lncRNA mediated gene regulation during abiotic stresses and reproductive development in legumes: in Long non-coding RNAs in plants: Roles in development and stress, Academic Press (Elsevier), (ISBN: 978-0-12-821452-7) https://doi.org/10.1016/B978-0-12-821452-7.00010-6
B Singh, S Mehta, SK Aggarwal, M Tiwari, SI Bhuyan, S Bhatia, and Md Aminul Islam (2019) Barley, Disease Resistance, and Molecular Breeding Approaches in Disease Resistance in Crop Plants, S. H. Wani (ed.) Springer Nature Switzerland AG, https://doi.org/10.1007/978-3-030-20728-1_11
Transcriptome Analysis in Chickpea (Cicer arietinum L.): Applications in Study of Gene Expression, Non-Coding RNA Prediction, and Molecular Marker Development (2017) Chandra Kant, Vimal Pandey, Subodh Verma, Manish Tiwari, Santosh Kumar and Sabhyata Bhatia http://dx.doi.org/10.5772/intechopen.69884
Bhatia S and Shokeen B (2009) Isolation of Microsatellites from Catharanthus roseus (L.) G. Don Using Enriched Libraries. In: Mohan Jain S and Saxena PK (eds) Methods in Molecular Biology-Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, vol. 547, Humana Press, a part of Springer Science + Business Media, pp 289-302
Varshney RK, Hoisington DA, Upadhyaya HD, Gaur PM, Nigam SN, Saxena K, Vadez V, Sethy NK, Bhatia S, Aruna R, Gowda MVC, Singh NK (2007) Molecular genetics and breeding of grain legume crops for the semi-arid tropics. In: Varshney R and Tuberosa R. (eds) Genomic Assisted Crop Improvement: Genomics Applications in Crops Vol 2, Springer publication, pp 207-241.
Lakshmikumaran M, Das S, Rajagopal J, Goswami J, Negi MS, Bhatia S (1998) Repeated DNA sequences in plants: Organization, Evolution and Applications. In: Gupta PK, Singh SP, Balyan HS, Sharma PC and Ramesh B (eds) Genetics and Biotechnology in Crop Improvement, Rastogi Publications, Meerut, India, pp. 63-93
Bhatia S and Shokeen B (2009) Isolation of Microsatellites from Catharanthus roseus (L.) G. Don Using Enriched Libraries. In: Mohan Jain S and Saxena PK (eds) Methods in Molecular Biology-Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, vol. 547, Humana Press, a part of Springer Science + Business Media, pp 289-302.

Varshney RK, Hoisington DA, Upadhyaya HD, Gaur PM, Nigam SN, Saxena K, Vadez V, Sethy NK, Bhatia S, Aruna R, Gowda MVC, Singh NK (2007) Molecular genetics and breeding of grain legume crops for the semi-arid tropics. In: Varshney R and Tuberosa R. (eds) Genomic Assisted Crop Improvement: Genomics Applications in Crops Vol 2, Springer publication, pp 207-241.

Lakshmikumaran M, Das S, Rajagopal J, Goswami J, Negi MS, Bhatia S (1998) Repeated DNA sequences in plants: Organization, Evolution and Applications. In: Gupta PK, Singh SP, Balyan HS, Sharma PC and Ramesh B (eds) Genetics and Biotechnology in Crop Improvement, Rastogi Publications, Meerut, India, pp. 63-93.
 Book: (Special Report) One

Lakshmikumaran M, and Bhatia S. 1996.  DNA fingerprinting of medicinal plants: A means to preserve valuable genetic resources.  RGICS Project No. 11. Rajiv Gandhi Institute for Contemporary Studies.  Rajiv Gandhi Foundation, New Delhi.