BRIC-National Institute of Plant Genome Research

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BRIC-National Institute of Plant Genome Research

An Autonomous Institute of Biotechnology Research and Innovation Council
Deptartment of Biotechnology, Ministry of Science and Technology
Government of India

Sowing seeds for a better tomorrow

Dr. Ashutosh Pandey

Scientist V

Profile

Research Area

Plant molecular biology, bioinformatics and phytochemistry to study the nutritional traits in crop plants (Banana and chickpea)

Research Interests

Understanding regulation of plant specialized metabolism and metabolic engineering of crop plants

Several plant specialized metabolites are of immense human interest owing to their health benefits. Certain secondary metabolites, for example flavonoids can be taken as neutraceuticals in diet. However, several commonly consumed foods are deficient in the content of these health beneficial compounds. It is therefore desirable to improve the content of such compounds in crop plants through application of genetic engineering.
My broad research focus is to understand molecular basis of plant specialized metabolism and to use developed knowledge in metabolic engineering of plants for enhancement of the content of health beneficial plant specialized metabolites. Being crop for major world population including India, banana and chickpea are an attractive target for metabolic engineering of health beneficial plant specialized metabolites. Release of genome sequence of both the crops have provided ample resource to understand aspects of specialized metabolism at molecular level. The major objective is to identify regulatory proteins which regulate the biosynthesis of different classes of flavonoids such as flavonol, anthocyanin and proanthocyanidnes. The identification of such transcription factors will be useful in developing transgenic value added banana enriched in health beneficial flavonoids. To achieve this objective, I employ tools of molecular biology, bioinformatics and phytochemistry. With this, my lab aims to develop value added crops enriched with health beneficial specialized metabolites.

Development of Value-added biomolecules through transformation of abundant plant biomass: (Waste to value)

Value added biomolecules has always remained on top priority for generation of bio-value in the abundant plant biomass, which otherwise turn into agro-industrial waste or by product in excessive quantity. There is huge gap in developing a product involving appropriate delivery system from biomolecule enriched plant biomass which is otherwise a waste. This requires innovations in development of improved biocatalysts, engineering of cell factories with modified metabolic efficiency, and nano-biotechnological tools to bioprocess the plentiful plant biomass. It is envisaged that product through this innovation could be of immense societal benefit especially for healthcare. Banana peel (BP) comprises about 30тАУ40% (w/w) of fresh fruit biomass. The fruit processing, packing, distribution and consumption, generates huge amount of waste or residual biomass. The residual wastes of this huge quantity, not only pose serious environmental risks, but also represent an immense loss of nutrients and bioactive compounds therein. We are exploring biotechnological solutions to utilize the abundant BP biomass for production of high-value biomolecules.My broad research focus is to understand molecular basis of plant specialized metabolism and to use developed knowledge in metabolic engineering of plants for enhancement of the content of health beneficial plant specialized metabolites. Being crop for major world population including India, banana and chickpea are an attractive target for metabolic engineering of health beneficial plant specialized metabolites. Release of genome sequence of both the crops have provided ample resource to understand aspects of specialized metabolism at molecular level. The major objective is to identify regulatory proteins which regulate the biosynthesis of different classes of flavonoids such as flavonol, anthocyanin and proanthocyanidnes. The identification of such transcription factors will be useful in developing transgenic value added banana enriched in health beneficial flavonoids. To achieve this objective, I employ tools of molecular biology, bioinformatics and phytochemistry. With this, my lab aims to develop value added crops enriched with health beneficial specialized metabolites.

Career

2024-present

Staff Scientist IV: National Institute of Plant Genome Research, New Delhi.

2021-2023

Staff Scientist III: National Institute of Plant Genome Research, New Delhi

2017-2020

Staff Scientist II: National Institute of Plant Genome Research, New Delhi

2016-2017

Alexander von Humboldt Fellow: CeBiTec, University of Bielefeld, Germany

2013-2016

Project Scientist: National Agri-Food Biotechnology Institute, Mohali, India

Awards & Honors

2023

INSA Associate Fellow: Indian National Science Academy (INSA), New Delhi

2019

Fellow: Alexander von Humboldt Foundation, Germany

2018

Member: Indian National Young Academy of Science (INYAS), New Delhi

2017

INSA Medal for Young Scientist: Indian National Science Academy (INSA), New Delhi

Current Members

Dr. Shivi Tyagi

Post Doc. Fellow (DBT-RA)

Dr. Sweta Bhambhani

Research Associate I (DST-SERB)

Jogindra Naik

Ph.D. Student (CSIR-SRF)

Samar Singh

Ph.D. Student (UGC-JRF)

Himani Chhatwal

Ph.D. Student (UGC-JRF)

Kumar Anchal

Ph.D. Student (DST-INSPIRE)

Biswaranjan Rout

NIPGR STRF (Project Associate)

Rajni Sharma

Project Fellow (JRF)

Niyaz Ahmed

Project Fellow (JRF)

Selected Research Article

Rajput, R., Tyagi, S., Kumar, A., Singh, S., Laxmi, A., Misra, P.,┬аPandey, A*. (2025) Cytokinin-mediated repression of anthocyanin biosynthesis in banana fruits.┬аThe Plant Journal┬аDOI┬аhttps://doi.org/10.1111/tpj.70267┬а122 (6), e70267┬а(*Corresponding Author)

Naik, J., Rajput, R., Stracke, R.,┬аPandey, A*. (2025) Heat-responsive MaHSF11 transcriptional activator positively regulates flavonol biosynthesis and flavonoid B-ring hydroxylation in banana┬а(Musa acuminata)┬аThe Plant Journal┬а1. 121, e70084 doi: 10.1111/tpj.70084┬а(*Corresponding Author)

Sinha, S., Sardar, A., Rai, D., Tripathi, A.K., Kothari. P., Rajput, R.,┬аPandey, A*., Trivedi, R*. (2025) Comparative assessment of flavonoid content of banana pulp and peel and their role in mitigating bone loss conditions and promoting osteoblast differentiation┬аFood & Function (Royal Society of Chemistry)┬аDOIhttps://doi.org/10.1039/D4FO04943H┬а(IF: 5.1) (*Corresponding Author)

Singh, S., Pal, L., Rajput, R., Chattopadhyay, D*., &┬аPandey, A*. (2024) CaLAP orchestrates anthocyanin biosynthesis in the seed coat of┬аCicer arietenum┬аPlanta┬а260(2):38. doi: 10.1007/s00425-024-04470-7┬а(*Corresponding Author)

Naik, J., Tyagi, S., Rajput, R., Kumar, P., Pucker, B., Bisht, N.C., Misra, P., Stracke, R.,┬аPandey, A*. (2024) Flavonols affect the interrelated glucosinolate and camalexin biosynthetic pathways in┬аArabidopsis thaliana.┬аJournal of Experimental Botany┬а75, 219-240┬а(*Corresponding Author)

Saxena, S., Pal, G.,┬аPandey, A*. (2023) Functional characterization of 2-oxoglutarate-dependent dioxygenase gene family in chickpea.┬аPlant Science┬а336, 111836┬а(*Corresponding Author)

Saxena, S., Pal, L., Naik, J., Singh, Y., Verma, P.K., Chattopadhyay, D.,┬аPandey, A.*┬а(2023) The R2R3-MYB-SG7 transcription factor CaMYB39 orchestrates surface phenylpropanoid metabolism and pathogen resistance in chickpea┬аNew Phytologist┬а┬аhttps://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18758┬а(*Corresponding Author)

Singh, S.K., Verma, S., Singh, K., Shree, A., Singh, R., Srivastava, V., Kumar, K., Pandey, A.,┬аVerma, P.K. (2023) The nuclear effector ArPEC25 from the necrotrophic fungus┬аAscochyta rabiei┬аtargets the chickpea transcription factor Ca╬▓LIM1a and negatively modulates lignin biosynthesis for host susceptibility┬аThe Plant Cell┬аhttps://doi.org/10.1093/plcell/koac372

Pal, L., Dwivedi, v., Gupta, S.K Saxena, S.,┬аPandey, A.*,┬аChattopadhyay D*. (2023) Biochemical analysis of anthocyanin and proanthocyanidin and their regulation in determining chickpea flower and seed coat colours,┬аJournal of Experimental Botany┬а74: 130-148.┬а(*Corresponding Author)

Rajput R, Naik J, Stracke R,┬аPandey A.*┬а(2022) Interplay between R2R3 MYB-type activators and repressors regulates proanthocyanidin biosynthesis in banana (Musa acuminata)┬аNew Phytologist┬а236:1108тАУ1127.┬а(*Corresponding Author)

Rajput, R., Tyagi, S., Naik, J., Pucker, B., Stracke, R.┬аPandey A.*┬а(2022) The R2R3-MYB gene family in┬аCicer arietinum: genome-wide identification and expression analysis leads to functional characterization of proanthocyanidin biosynthesis regulators in the seed coat┬аPlanta┬а256:67┬а(*Corresponding Author)

Gani U, Nautiyal AK, Kundan M, Rout B,┬аPandey A.,┬аMisra P. (2022) Two homeologous MATE transporter genes,┬аNtMATE21┬аand┬аNtMATE22, are involved in the modulation of plant growth and flavonol transport in┬аNicotiana tabacum.┬аJournal of Experimental Botany┬а73:6186-6206

Deb, D., Basak, S., Kar, T., Narsaria, U., Castiglione, F., Paul, A.,┬аPandey A.*, Srivastava, A.P.*. (2021) Immunoinformatics based designing a multi-epitope vaccine against pathogenic┬аChandipura vesiculovirus┬аJournal of Cellular Biochemistry┬а123:322-346.┬а(*Corresponding Author)

Naik, J., Rajput, R., Pucker, B., Stracke, R.,┬аPandey A.*┬а(2021) The R2R3-MYB transcription factor┬аMtMYB134┬аorchestrates flavonol biosynthesis in┬аMedicago truncatrula.┬аPlant Molecular Biology┬а106:157-172.┬а(*Corresponding Author)

Chatterjee, A., Paul, A., Unnati, G.H., Ruchika, Biswas, T., Kar, T., Basak, S., Mishra, N.,┬аPandey, A.*,┬аSrivastava, A.P.* (2020)1 MAPK cascade gene family in┬аCamellia sinensis: In-silico identification, expression profiles and regulatory network analysis.┬аBMC Genomics┬а21:613┬а(*Corresponding Author)

Kaur, N., Alok, A., Shivani, Kumar, P., Kaur, N., Awasthi, P., Chaturvedi, S., Pandey, P.,┬аPandey, A.,┬аPandey, A.K., Tiwari, S. (2020) CRISPR/Cas9 directed editing of lycopene epsilon-cyclase modulates metabolic flux for ╬▓-carotene biosynthesis in banana fruit.┬аMetabolic Engineering. 59:76-86

Pandey, A.*,┬аAlok, A., Lakhwani, D., Singh, J., Asif, M.H., Trivedi, P.K.* (2016) Genome-wide expression analysis and metabolite profiling elucidate transcriptional regulation of flavonoid biosynthesis and modulation under abiotic Stresses in Banana.┬аScientific Reports┬а6:31361.┬а(*Corresponding Author)

Sharma, D., Tiwari, M.,┬аPandey, A.,┬аBhatia, C., Sharma, A. Trivedi, P.K. (2016) MicroRNA858 is a potential regulator of phenylpropanoid pathway and plant development in Arabidopsis.┬аPlant Physiology┬а17, 944-959.

Pandey, A.*,┬аMisra, P., Alok, A., Kaur, N., Sharma, S., Lakhwani, D., Asif, M.H., Tiwari, S., Trivedi, P.K.*. Genome wide identification and expression analysis of Homeodomain leucine zipper subfamily IV (HDZ IV) gene family from┬аMusa accuminata.┬аFrontiers in Plant Science┬а1;7:20.┬а(*Corresponding Author)

Pandey, A.,┬аMisra, P., Khan, M.P., Swarnker, G., Tewari, M.C., Bhambhani, S., Trivedi, R., Chattopadhyay, N. Trivedi, P.K. (2014) Coexpression of Arabidopsis transcription factor,┬аAtMYB12, and soybean isoflavone synthase,┬аGmIFS1,┬аgenes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity.┬аPlant Biotechnology Journal┬а12, 69-80

Misra, P., Pandey, A., Tiwari, M., Chandrashekhar, K., Siddhu, O.P., Asif, M.H., Chakrabarty, D., Singh, P.K., Nath, P., Trivedi, P.K. Tuli, R. (2010) Modulation of transcriptome and metabolome by┬аAtMYB12┬аtranscription factor leads to insect tolerance.┬аPlant Physiology,┬а152, 2258-2268

Selected Review Articles

Rajput R., Naik J., Misra P., Trivedi P.K.*,┬аPandey A.*┬а(2022) Gene pyramiding in transgenic plant development: Approaches and challenges.┬аJournal of Plant Growth Regulation┬аhttps://doi.org/10.1007/s00344-022-10760-9┬а(*Corresponding Author)

Naik J, Misra P, Trivedi P.K.*,┬аPandey A.*┬а(2022) Molecular components associated with the regulation of flavonoid biosynthesis┬аPlant Science┬аDOI┬аhttps://doi.org/10.1016/j.plantsci.2022.111196┬а(*Corresponding Author)

Pal, G., Saxena, S., Kumar, K., Verma, A., Sahu, P.K.,┬аPandey, A.,┬аWhite, J.F., Verma, S.K. (2022) Endophytic Burkholderia: Multifunctional roles in plant growth promotion and stress tolerance┬аMicrobiological Research┬аDOI┬аhttps://doi.org/10.1016/j.micres.2022.127201

Alok, A., Chauhan, H., Upadhyay, S.K.,┬аPandey, A.,┬аKumar, J., Singh, K. (2021) Compendium of plant specific CRISPR vectors and their technical advantages.┬аLife,┬а11, 1021.https://doi.org/10.3390/life11101021

Tiwari, M.*, Trivedi, P.K.,┬аPandey A.*┬а(2020) Harnessing gene editing potential of CRISPR-Cas protein for improving agronomic traits in staple crops.┬аFood and Energy Security┬аDOI:10.1002/fes3.258┬а(*Corresponding Author)

Book Chapter

Singh, S., Naik, J.,┬аPandey, A*.┬а(2022) Genetics of Plant Organelles: Plastid and Mitochondrial Genomes In: Singh, R.L., Mondal, S., Parihar, A., Singh, P.K.- Plant Genomics for Sustainable Agriculture┬аSpringer Nature Singapore:┬аpp 313-330 DOI:┬аhttps://doi.org/10.1007/978-981-16-6974-3_12

Ruchika, Naik J,┬аPandey A┬а(2019) Synthetic Metabolism and Its Significance in Agriculture. In: Current Developments in Biotechnology and Bioengineering Synthetic Biology, Cell Engineering and Bioprocessing Technologies pp 365-391.┬аElsevier.┬аISBN: 9780444640864

Singh SP, Upadhyay SK,┬аPandey A,┬аKumar S (2019) Molecular Approaches in Plant Biology and Environmental Challenges Editors: Singh SP, Upadhyay SK,┬аPandey A,┬аKumar S (Eds.) pp 1-5.┬аSpringer-Verlag Ltd.┬аSingapore: ISBN 978-981-15-0690-1

Kaur N, Shivani,┬аPandey A,┬аand Tiwari S. (2016) Provitamin A enrichment for tackling malnutrition. In: Mohandas S, Ravishankar KV (eds) Banana: Genomics and transgenic approaches for genetic improvement.┬аSpringer-Verlag Ltd. Singapore: pp 277тАУ300. DOI 10.1007/978-981-10-1585-4_19

Book edited

Molecular Approaches in Plant Biology and Environmental Challenges Editors: Singh SP, Upadhyay SK,┬аPandey A,┬аKumar S (Eds.)┬аSpringer-Verlag Ltd.┬аSingapore: ISBN 978-981-15-0690-1

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