National Institute of Plant Genome Research
Digital India     
 
Scientist in-charge: Dr. Subhra Chakraborty, Dr. Manoj Majee & Dr. Jitendra Thakur
Overview
Proteomics is a very powerful and promising approach to study proteins involved in various biological processes. New analytical techniques along with the growing availability of genomic sequence information make it possible to systematically analyze in parallel numerous proteins in a cell or tissue expressed under a certain condition. Proteomics facility at the National Institute of Plant Genome Research uses mass spectrometry as the key technology for the qualitative and quantitative characterization of proteins. The facility, funded by the Department of Biotechnology, Govt. of India, started in 2007 with the aim to initiate, coordinate and facilitate proteomics research activities. The facility has several equipments and experience for proteomics activities at its disposal. The service of the facility is available to internal and external users.
Activities
The activities of this facility are mainly focused on the identification of proteins and quantification of the targeted peptides.
Protein samples supplied as SDS-PAGE gel bands and stained with MS-compatible dye or in solution, are subjected to tryptic digestion and peptides extracted according to standard protocols. Peptides are analyzed by electrospray ionization time-of-flight mass spectrometry using Sciex TripleTOF 6600 coupled online with ekspert nanoLC 425 or Sciex CESI 8000 Plus system. Peptides are identified by searching the peak list generated by Analyst  software against the MASCOT (http://www.matrixscience.com) search engine or Protein Pilot software. Quantitative analysis of the targeted peptides are performed using the Sciex QTRAP 6500+ coupled online with Sciex Exion LC system.
Instrumentation
 
 
Liquid Chromatography-Mass spectrometry- LC- MS/MS (QTRAP 6500+) Eksigent NanoLC 400-TripleTOF® 6600 quadrupole time-of-flight (QTOF)
 
1) Price List
2) Sample submission form- NIPGR Internal users
3) Sample submission form- External users