The five-day workshop will provide both hands-on instrument use and in-depth tutorials for understanding mass spectrometry workflows, sample handling, data collection and data analysis using Sciex instrumentation, Sciex software platforms including One Omics and the Institute for Systems Biology Trans-Proteomic pipeline. The course will cover experimental design, sample preparation, mass spectrometer optimization, data acquisition, analysis and interpretation, as well as database setup and query. Data analysis will include capabilities to perform both targeted quantitative proteomics (MRM) as well as SWATH analysis.