{"id":2719,"date":"2025-09-10T10:19:15","date_gmt":"2025-09-10T10:19:15","guid":{"rendered":"https:\/\/nipgr.ac.in\/nipgrv4\/?page_id=2719"},"modified":"2025-10-01T08:55:42","modified_gmt":"2025-10-01T08:55:42","slug":"proteomics","status":"publish","type":"page","link":"https:\/\/nipgr.ac.in\/nipgrv4\/proteomics\/","title":{"rendered":"PROTEOMICS"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2719\" class=\"elementor elementor-2719\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6dec5950 e-con-full e-flex e-con e-parent\" data-id=\"6dec5950\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t<div class=\"elementor-element elementor-element-66f21b7e e-flex e-con-boxed e-con e-child\" data-id=\"66f21b7e\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-55ccda19 elementor-widget elementor-widget-heading\" data-id=\"55ccda19\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Proteomics<\/h2>\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-62c5364d e-flex e-con-boxed e-con e-parent\" data-id=\"62c5364d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-56297102 e-con-full e-flex e-con e-child\" data-id=\"56297102\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-750dc11 elementor-widget elementor-widget-heading\" data-id=\"750dc11\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Overview<\/h2>\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-2c88a46 e-con-full e-flex e-con e-child\" data-id=\"2c88a46\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3963759 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"3963759\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-657471ae elementor-widget elementor-widget-text-editor\" data-id=\"657471ae\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p>Proteomics is a very powerful and promising approach to study proteins involved in various biological processes. New analytical techniques along with the growing availability of genomic sequence information make it possible to systematically analyze in parallel numerous proteins in a cell or tissue expressed under a certain condition. Proteomics facility at the National Institute of Plant Genome Research uses mass spectrometry as the key technology for the qualitative and quantitative characterization of proteins. The facility, funded by the Department of Biotechnology, Govt. of India, started in 2007 with the aim to initiate, coordinate and facilitate proteomics research activities. The facility has several equipments and experience for proteomics activities at its disposal. The service of the facility is available to internal and external users.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d830116 elementor-widget elementor-widget-heading\" data-id=\"d830116\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Activities<\/h2>\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-4905c1b e-con-full e-flex e-con e-child\" data-id=\"4905c1b\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ad25cc4 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"ad25cc4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ae793c1 elementor-widget elementor-widget-text-editor\" data-id=\"ae793c1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p>The activities of this facility are mainly focused on the identification of proteins and quantification of the targeted peptides.<\/p><p>Protein samples supplied as SDS-PAGE gel bands and stained with MS-compatible dye or in solution, are subjected to tryptic digestion and peptides extracted according to standard protocols. Peptides are analyzed by electrospray ionization time-of-flight mass spectrometry using Sciex TripleTOF 6600 coupled online with ekspert nanoLC 425 or Sciex CESI 8000 Plus system. Peptides are identified by searching the peak list generated by Analyst\u00a0 software against the MASCOT (<u><a href=\"http:\/\/www.matrixscience.com\/\" target=\"_blank\" rel=\"noopener\">http:\/\/www.matrixscience.com<\/a><\/u>) search engine or Protein Pilot software. Quantitative analysis of the targeted peptides are performed using the Sciex QTRAP 6500+ coupled online with Sciex Exion LC system.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e7fb7f1 elementor-widget elementor-widget-heading\" data-id=\"e7fb7f1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Instrumentation\n<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-19f211e elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"19f211e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-f598c76 e-con-full e-flex e-con e-child\" data-id=\"f598c76\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t<div class=\"elementor-element elementor-element-791677f e-con-full e-flex e-con e-child\" data-id=\"791677f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-95ca6c2 elementor-widget elementor-widget-image\" data-id=\"95ca6c2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"386\" height=\"346\" src=\"https:\/\/nipgr.ac.in\/nipgrv4\/wp-content\/uploads\/2025\/09\/protemics1-1.png\" class=\"attachment-large size-large wp-image-2725\" alt=\"\" srcset=\"https:\/\/nipgr.ac.in\/nipgrv4\/wp-content\/uploads\/2025\/09\/protemics1-1.png 386w, https:\/\/nipgr.ac.in\/nipgrv4\/wp-content\/uploads\/2025\/09\/protemics1-1-300x269.png 300w\" sizes=\"(max-width: 386px) 100vw, 386px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dd38197 elementor-widget elementor-widget-heading\" data-id=\"dd38197\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Exion LC-QTRAP 6500+<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7a336ff e-con-full e-flex e-con e-child\" data-id=\"7a336ff\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f11a4b8 elementor-widget elementor-widget-image\" data-id=\"f11a4b8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"386\" height=\"346\" src=\"https:\/\/nipgr.ac.in\/nipgrv4\/wp-content\/uploads\/2025\/09\/protemics2.png\" class=\"attachment-large size-large wp-image-2724\" alt=\"\" srcset=\"https:\/\/nipgr.ac.in\/nipgrv4\/wp-content\/uploads\/2025\/09\/protemics2.png 386w, https:\/\/nipgr.ac.in\/nipgrv4\/wp-content\/uploads\/2025\/09\/protemics2-300x269.png 300w\" sizes=\"(max-width: 386px) 100vw, 386px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-620f38d elementor-widget elementor-widget-heading\" data-id=\"620f38d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Eksigent ekspert nanoLC 425-TripleTOF 6600<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c8d88f9 elementor-widget elementor-widget-heading\" data-id=\"c8d88f9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Contact<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dfdc9b6 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"dfdc9b6\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0d1d416 e-con-full e-flex e-con e-child\" data-id=\"0d1d416\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t<div class=\"elementor-element elementor-element-9ba4461 e-con-full e-flex e-con e-child\" data-id=\"9ba4461\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-14bb990 elementor-widget elementor-widget-heading\" data-id=\"14bb990\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Scientists in-charge:<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-12998ca elementor-widget elementor-widget-text-editor\" data-id=\"12998ca\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p>Dr. Manoj Majee<br \/>Dr. Prabhakaran Soundararajan<br \/>Dr. Divya Mishra<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1d26120 e-con-full e-flex e-con e-child\" data-id=\"1d26120\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-93c21c3 elementor-widget elementor-widget-heading\" data-id=\"93c21c3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Technical in-charge:<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6e2f643 elementor-widget elementor-widget-text-editor\" data-id=\"6e2f643\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t\t\t\t\t\t<p>Ms. Umamaheswari Rajamani (STO)<br \/>Mr. Naga Vara Prasad G (STO)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0cf4831 elementor-view-default elementor-position-block-start elementor-mobile-position-block-start elementor-widget elementor-widget-icon-box\" data-id=\"0cf4831\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-box.default\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon-box-wrapper\">\n\n\t\t\t\t\t\t<div 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