Total Credit : 3 Total Number of Lectures : 48 Course In-charge:Dr. Manoj Majee & Dr. Naveen Chandra Bisht |
Fundamental knowledge of Molecular Cell Biology and Genetics is the basis of unraveling nature's secret hidden in biological system. The students are expected to refresh their knowledge on cellular biology, molecular biology and genetics through this course. Emphasis will be given to explain the topics giving relevant examples that might help Ph.D. students in designing their experiments and interpretation of their observations. |
1. Cellular Biology (16 lectures) |
- Constituents of Plant Cells: Extracellular matrix, Cytoskeleton, and Organelles.
- Cell Cycle Regulation: Phases of cell cycle, Restriction and check point, Cell division and cell growth, Cell cycle progression.
- Enzyme Function.
- Protein Turnover: Biosynthesis and degradation.
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2. Molecular Biology (12 lectures) |
- Genetic Material: Genome organization, DNA replication and recombination, Source of genetic variation (natural and induced).
- Gene Expression: Transcription, Cis-acting elements and transcription factors, RNA editing and processing.
- Protein Targeting and Trafficking: Protein trafficking (classical and non-classical pathways), ER and Golgi dynamics, Protein sorting and trafficking, Dynamics of membrane-bound protein, Mechanism of protein secretion.
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3. Genetics (20 Lectures) |
- Law of Inheritance: Mendelian principles, Concept of dominance, Segregation and independent assortment, Codominance, Incomplete dominance, Gene interactions, Pleiotropy, Linkage and crossing over.
- Allelic and Non-allelic Interaction: Concept of allele, Lethal alleles, Multiple alleles, Test of allelism, Complementation and epistatsis.
- Mutation: Types of mutation, Repair mechanism, Role in genetic analysis and evolution.
- Cytoplasmic Inheritance: Basis and mechanism, Role of organellar genes.
- Recombination: Homologous and non-homologous recombination including transposition.
- Structural and Numerical Alterations of Chromosomes: Polyploidy, Aneuploidy, Chromosomal rearrangements – deletion, duplication, inversion and translocation.
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Suggested Reading |
- Nelson DL and Cox MM (2008) Lehninger “Principles of Biochemistry”. Fifth Edition, Freeman & Co Ltd., NY, USA.
- Alberts B, Johnson A, Lewis J, Raff M, Roberts K and Walter P (2008) Molecular Biology of the Cell. Fifth Edition, Garland Science, NY, USA.
- Voet D and Voet JG (2011) Biochemistry. Fourth Edition, John Wiley & Sons, NY, USA.
- Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D and Darnell J (2012) Molecular Cell Biology. Seventh Edition, Taylor & Francis Publishers, NY, USA.
- Krebs JE, Goldstein ES and Kilpatrick ST (2011) Lewin’s Genes X. Tenth Edition, Jones & Bartlett Publishers, MA, USA.
- Latchman DS (2007) Gene Regulation. Fifth Edition, Taylor & Francis Publishers, NY, USA.
- Brown TA (2007) Genomes. Third Edition, Garland Science, NY, USA.
- Snustad DP and Simmons MJ (2012) Principles of Genetics. Sixth Edition, John Wiley & Sons, NY, USA.
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