Kompetitive allele specific PCR (KASP) is a single-step homogenous, fluorescence-based genotyping variant of polymerase chain reaction used for the detection of variants between parents and progenies. KASP do bi-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (Indels) at specific loci.

The KASP genotyping method is highly sensitive, cost-effective, very shorter turnaround time to receive the results and based on independent assessment it has very high genotyping accuracy rate > 99.8%. This method is highly flexible than any other genotyping methods as it can be used when there are large number of SNPs in a few samples or when there are few SNPs in large number of samples. KASP has been extensively used in massive SNP genotyping studies and molecular markers-assisted breeding.

Applications

• Quality control (QC): maintaining the purity of inbred line
• Quantitative trait locus (QTL) mapping,
• Marker-assisted selection (MARS)
• Allele mining.
• Assessment of line purity

Project Workflow
At NGGF we care for your samples from the start to the delivery of result with a project specific detailed report. Our highly experienced scientist and laboratory professionals follow strict quality procedures to ensure the integrity of your results.

For enquiry please contact us at:

• Phone :91-11-26735-181
• E-mail : infonggf@nipgr.ac.in